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Again, this is not the case with osteoarthritis of the big toe which will become stiffer as time passes

Clin Exp Immunol 72:284287 Tye-Din JA, Daveson AJM, Ee HC, Goel G, MacDougall J, Acaster S, Goldstein KE, Dzuris JL, Neff KM, Truitt KE et al (2019) Elevated serum interleukin-2 after gluten correlates with symptoms and is a potential diagnostic biomarker for coeliac disease

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After 24 h, the medium was removed, 4% paraformaldehyde (PFA) was added for 30 min for cell fixation and the cells were stained with crystal violet at a concentration of 0.05% for 15 min